Biotin was first identified as a nutritive requirement of yeast. Originally called vitamin H, it was isolated in pure form in 1935; its structure was established in 1942, after it had been shown to be required by animals. Evidence for the necessity of biotin appeared with the discovery in 1927 that the addition of uncooked egg white to a diet that is otherwise adequate produces toxicity and disease. This is because egg white contains a specific protein, avidin, that combines with biotin and thus prevents its absorption. In practice, biotin deficiency results only from the prolonged consumption of an exceptionally large number of uncooked egg whites; symptoms include dermatitis and hair loss.