Goodall, who was interested in animal behaviour from an early age, left school at age 18. She worked as a secretary and as a film production assistant until she gained passage to Africa. Once there, Goodall began assisting paleontologist and anthropologist Louis Leakey (Leakey, Louis S.B.). Her association with Leakey led eventually to her establishment in June 1960 of a camp in the Gombe Stream Game Reserve (now a national park) so that she could observe the behaviour of chimpanzees in the region. In 1964 she married a Dutch photographer who had been sent in 1962 to Tanzania to film her work (later they divorced). The University of Cambridge in 1965 awarded Goodall a Ph.D. in ethology; she was one of very few candidates to receive a Ph.D. without having first possessed an A.B. degree. Except for short periods of absence, Goodall and her family remained in Gombe until 1975, often directing the fieldwork of other doctoral candidates. In 1977 she cofounded the Jane Goodall Institute for Wildlife Research, Education, and Conservation in California; the centre later moved its headquarters to Washington, D.C.

Over the years Goodall was able to correct a number of misunderstandings about chimpanzees. She found, for example, that the animals are omnivorous, not vegetarian; that they are capable of making and using tools; and, in short, that they have a set of hitherto unrecognized complex and highly developed social behaviours. Goodall wrote a number of books and articles about various aspects of her work, notably In the Shadow of Man (1971). She summarized her years of observation in The Chimpanzees of Gombe: Patterns of Behavior (1986). Goodall continued to write and lecture about environmental and conservation issues into the early 21st century. The recipient of numerous honours, she was created Dame of the British Empire in 2003.