Her family immigrated to the United States in 1870, and at the age of 11 she toured the Midwest in a juvenile production of Gilbert and Sullivan's H.M.S. Pinafore. Ten years later she made her New York debut. Her first financially successful role was that of the sister of Henry VIII in When Knighthood Was in Flower, a play she also directed. She dominated this immensely popular 1900 production, adapted especially for her from Charles Major's novel; it ran for two seasons. In 1904 she teamed with Edward Hugh Sothern (Sothern, Edward Hugh) (whom she married in 1911), and together they became the leading couple of Shakespearean actors of their day. Among Marlowe's greatest roles were Viola in Shakespeare's Twelfth Night and Julia in James Sheridan Knowles's play The Hunchback. She retired because of poor health in 1916 but appeared on stage sporadically until 1924.