microorganisms capable of transforming atmospheric nitrogen into fixed nitrogen, inorganic compounds usable by plants. More than 90 percent of all nitrogen fixation is effected by them.

Two kinds of nitrogen fixers are recognized: free-living (non-symbiotic) bacteria, including the cyanobacteria (or blue-green algae) Anabaena and Nostoc and such genera as Azotobacter, Beijerinckia, and Clostridium; and mutualistic (symbiotic) bacteria such as Rhizobium, associated with leguminous plants, and Spirillum lipoferum, associated with cereal grasses.

The symbiotic nitrogen-fixing bacteria invade the root hairs of host plants, where they multiply and stimulate formation of root nodules, enlargements of plant cells and bacteria in intimate association. Within the nodules the bacteria convert free nitrogen to nitrates, which the host plant utilizes for its development. To insure sufficient nodule formation and optimum growth of legumes (e.g., alfalfa, beans, clovers, peas, soybeans), seeds are usually inoculated with commercial cultures of appropriate Rhizobium species, especially in soils poor or lacking in the required bacterium. See also nitrogen cycle.