king of Judah (c. 640–609 BC), who set in motion a reformation that bears his name and that left an indelible mark on Israel's religious traditions (2 Kings 22–23:30).

Josiah was the grandson of Manasseh, king of Judah, and ascended the throne at age eight after the assassination of his father, Amon, in 641. For a century, ever since Ahaz, Judah had been a vassal of the Assyrian empire. Imperial policy imposed alien cults on Judah that suppressed or obscured the Israelite religious identity. After the death of King Ashurbanipal, the Assyrian empire fell into chaos; it could no longer assert its authority in Jerusalem (Jerusalem, Temple of). Egypt also was weak, and Judah thus obtained an unusual degree of independence from foreign powers. About 621 Josiah launched a program of national renewal, centred on the Temple in Jerusalem. A book believed to have contained provisions relating to covenantal traditions of premonarchic times deeply impressed him and gave a decisive turn to his reforms. The Temple was purged of all foreign cults and dedicated wholly to the worship of Yahweh, and all local sanctuaries were abolished, sacrifice being concentrated at Jerusalem.

In Assyria, Babylonia, which had long been a restive province, led a coalition that sacked Nineveh. The empire was in desperate straits; the Babylonians seemed about to displace it. Hoping to keep Mesopotamia divided, Necho (Necho II), the Egyptian pharaoh, set out to aid the hard-pressed Assyrians. He landed a force on the territory of the northern kingdom of Israel. King Josiah had hopes of a reunification of Judah and Israel, making the latter territory part of his own realm under the aegis of Babylonia. Consequently he challenged the pharaoh to battle; but it is reported that “Necho slew him at Megiddo, when he saw him” (2 Kings 23:29). Soon thereafter Assyria was completely eliminated, the Egyptians retreated, and Josiah's son, Jehoiakim, whom Necho had placed on the throne of Judah as a vassal, had to submit to Babylonia, the new Mesopotamian empire.